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Addgene inc ferritin
Ferritin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/frb+mcherry+fth1/FRB-mCherry-FTH1+(Plasmid+%23100749)/pmc12547712-385-0-3
Average 93 stars, based on 4 article reviews
ferritin - by Bioz Stars, 2026-09
93/100 stars

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Transfection:

Article Title: Cryo-electron tomography pipeline for plasma membranes
Article Snippet: HEK293 WT cells were purchased from ATCC (CRL-3216) and maintained in Eagle’s minimum essential medium (EMEM, Fisher Scientific 502382632) supplemented with 10% fetal bovine serum (FBS). .. For FerriTag experiments, HEK293 cells were transfected with FTL (Ferritin light chain, Addgene plasmid # 100750), FRB-mCherry-FTH1 (Ferritin Heavy Chain, Addgene Plasmid #100749), and one of either HIP1R-GFP-FKBP (Addgene Plasmid #100752) or GFP-FKBP-Lca (Addgene Plasmid #59353) [ ] using the Lonza Nucleofector and kit V (Lonza #VCA-1003) immediately prior to coverslip seeding. ..

Article Title: Cryo-electron tomography pipeline for plasma membranes
Article Snippet: HEK293, human (female, unknown ethnicity) embryonic kidney, WT cells were purchased from ATCC (CRL-3216) and maintained in Eagle’s minimum essential medium (EMEM, Fisher Scientific 502382632) supplemented with 10% fetal bovine serum (FBS). .. For FerriTag experiments, HEK293 cells were transfected with FTL (Ferritin light chain, Addgene plasmid # 100750), FRB-mCherry-FTH1 (Ferritin Heavy Chain, Addgene Plasmid #100749), and one of either HIP1R-GFP-FKBP (Addgene Plasmid #100752) or GFP-FKBP-LCa (Addgene Plasmid #59353) using the Lonza Nucleofector and kit V (Lonza #VCA-1003) immediately prior to coverslip seeding (western blot, Fig. ). ..

Plasmid Preparation:

Article Title: Cryo-electron tomography pipeline for plasma membranes
Article Snippet: HEK293 WT cells were purchased from ATCC (CRL-3216) and maintained in Eagle’s minimum essential medium (EMEM, Fisher Scientific 502382632) supplemented with 10% fetal bovine serum (FBS). .. For FerriTag experiments, HEK293 cells were transfected with FTL (Ferritin light chain, Addgene plasmid # 100750), FRB-mCherry-FTH1 (Ferritin Heavy Chain, Addgene Plasmid #100749), and one of either HIP1R-GFP-FKBP (Addgene Plasmid #100752) or GFP-FKBP-Lca (Addgene Plasmid #59353) [ ] using the Lonza Nucleofector and kit V (Lonza #VCA-1003) immediately prior to coverslip seeding. ..

Article Title: Cryo-electron tomography pipeline for plasma membranes.
Article Snippet: HEK293, human (female, unknown ethnicity) embryonic kidney, WT cells were purchased from ATCC (CRL-3216) and maintained in Eagle’s minimum essential medium (EMEM, Fisher Scientific 502382632) supplemented with 10% fetal bovine serum (FBS). .. For FerriTag experiments, HEK293 cellswere transfectedwith FTL (Ferritin light chain, Addgeneplasmid# 100750), FRB-mCherry-FTH1 (Ferritin Heavy Chain, Addgene Plasmid #100749), and one of either HIP1R-GFP-FKBP (Addgene Plasmid #100752) or GFP-FKBP-LCa (Addgene Plasmid #59353)16 using the Lonza Nucleofector and kit V (Lonza #VCA-1003) immediately prior to coverslip seeding (western blot, Fig. S11a, b). ..

Article Title: Cryo-electron tomography pipeline for plasma membranes
Article Snippet: HEK293, human (female, unknown ethnicity) embryonic kidney, WT cells were purchased from ATCC (CRL-3216) and maintained in Eagle’s minimum essential medium (EMEM, Fisher Scientific 502382632) supplemented with 10% fetal bovine serum (FBS). .. For FerriTag experiments, HEK293 cells were transfected with FTL (Ferritin light chain, Addgene plasmid # 100750), FRB-mCherry-FTH1 (Ferritin Heavy Chain, Addgene Plasmid #100749), and one of either HIP1R-GFP-FKBP (Addgene Plasmid #100752) or GFP-FKBP-LCa (Addgene Plasmid #59353) using the Lonza Nucleofector and kit V (Lonza #VCA-1003) immediately prior to coverslip seeding (western blot, Fig. ). ..

Western Blot:

Article Title: Cryo-electron tomography pipeline for plasma membranes.
Article Snippet: HEK293, human (female, unknown ethnicity) embryonic kidney, WT cells were purchased from ATCC (CRL-3216) and maintained in Eagle’s minimum essential medium (EMEM, Fisher Scientific 502382632) supplemented with 10% fetal bovine serum (FBS). .. For FerriTag experiments, HEK293 cellswere transfectedwith FTL (Ferritin light chain, Addgeneplasmid# 100750), FRB-mCherry-FTH1 (Ferritin Heavy Chain, Addgene Plasmid #100749), and one of either HIP1R-GFP-FKBP (Addgene Plasmid #100752) or GFP-FKBP-LCa (Addgene Plasmid #59353)16 using the Lonza Nucleofector and kit V (Lonza #VCA-1003) immediately prior to coverslip seeding (western blot, Fig. S11a, b). ..

Article Title: Cryo-electron tomography pipeline for plasma membranes
Article Snippet: HEK293, human (female, unknown ethnicity) embryonic kidney, WT cells were purchased from ATCC (CRL-3216) and maintained in Eagle’s minimum essential medium (EMEM, Fisher Scientific 502382632) supplemented with 10% fetal bovine serum (FBS). .. For FerriTag experiments, HEK293 cells were transfected with FTL (Ferritin light chain, Addgene plasmid # 100750), FRB-mCherry-FTH1 (Ferritin Heavy Chain, Addgene Plasmid #100749), and one of either HIP1R-GFP-FKBP (Addgene Plasmid #100752) or GFP-FKBP-LCa (Addgene Plasmid #59353) using the Lonza Nucleofector and kit V (Lonza #VCA-1003) immediately prior to coverslip seeding (western blot, Fig. ). ..

Expressing:

Article Title: FerriTag is a new genetically-encoded inducible tag for correlative light-electron microscopy
Article Snippet: .. For expression of FTL only, human ferritin light polypeptide cDNA (IMAGE clone: 2905327) was amplified by PCR and inserted into pEGFP-C1, removing EGFP, via Age I- Xho I. Plasmids to express FRB-mCherry-FTH1 and FTL are available from Addgene (100749 and 100750, respectively). ..

Amplification:

Article Title: FerriTag is a new genetically-encoded inducible tag for correlative light-electron microscopy
Article Snippet: .. For expression of FTL only, human ferritin light polypeptide cDNA (IMAGE clone: 2905327) was amplified by PCR and inserted into pEGFP-C1, removing EGFP, via Age I- Xho I. Plasmids to express FRB-mCherry-FTH1 and FTL are available from Addgene (100749 and 100750, respectively). ..

Polymerase Chain Reaction:

Article Title: FerriTag is a new genetically-encoded inducible tag for correlative light-electron microscopy
Article Snippet: .. For expression of FTL only, human ferritin light polypeptide cDNA (IMAGE clone: 2905327) was amplified by PCR and inserted into pEGFP-C1, removing EGFP, via Age I- Xho I. Plasmids to express FRB-mCherry-FTH1 and FTL are available from Addgene (100749 and 100750, respectively). ..

other:

Article Title: Coiled-coil interactions drive the ectopic condensation of overexpressed MAD1 to promote mitotic slippage in cancer cells
Article Snippet: FRB-mCherry-FTH1 , Foijer et al. , Addgene 63702.



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Image Search Results


HeLa cells expressing mCherry-eDHFR-MAD1, Halo-GFP-HOTag3, and miRFP670-H2B were arrested in mitosis with nocodazole. No dimerizer was added. Scale bar, 5 μm.

Journal: iScience

Article Title: Coiled-coil interactions drive the ectopic condensation of overexpressed MAD1 to promote mitotic slippage in cancer cells

doi: 10.1016/j.isci.2025.113638

Figure Lengend Snippet: HeLa cells expressing mCherry-eDHFR-MAD1, Halo-GFP-HOTag3, and miRFP670-H2B were arrested in mitosis with nocodazole. No dimerizer was added. Scale bar, 5 μm.

Article Snippet: FRB-mCherry-FTH1 , Foijer et al. , Addgene 63702.

Techniques:

HeLa cells expressing mCherry-eDHFR-MAD1, Halo-GFP-HOTag3, and miRFP670-H2B were arrested in mitosis with nocodazole. Dimerizer was added after the first time point. Scale bar, 5 μm.

Journal: iScience

Article Title: Coiled-coil interactions drive the ectopic condensation of overexpressed MAD1 to promote mitotic slippage in cancer cells

doi: 10.1016/j.isci.2025.113638

Figure Lengend Snippet: HeLa cells expressing mCherry-eDHFR-MAD1, Halo-GFP-HOTag3, and miRFP670-H2B were arrested in mitosis with nocodazole. Dimerizer was added after the first time point. Scale bar, 5 μm.

Article Snippet: FRB-mCherry-FTH1 , Foijer et al. , Addgene 63702.

Techniques: